The options of Graphene Oxide (GO) might produce sudden interactions with ecosystem, triggering unfavorable results on biota. On this work we evaluated the results of two totally different GO samples (chemically synthesized GOC and electrochemically synthesized GOE) on Lemna gibba L., after incubation with totally different concentrations over a interval of 21 days. The stress situations had been evidenced by the measurements of frond quantity, recent weight, dry weight, dry/recent weight ratio, and inhibition of development fee. Moreover, a morphological characterization was additionally carried out to confirm the mechanical results on L. gibba vegetation of those two GO. Outcomes demonstrated that the smallest GOE nano sheets had been very properly dispersed on L. gibba fronds than GOC. This impact might be defined contemplating the decrease GOE functionalization diploma and, in consequence, a much less electrostatic adhesion amongst nanosheets. The presence of electrically charged functionalities primarily offers electrostatic interactions with vegetation inflicting development inhibition as proven by GOC.