Ligand-mediated interface management has been broadly utilized as a strong software in setting up refined nanocomposites. Nonetheless, the resultant morphologies are often restricted to stable buildings. Now, a facile spatially controllable ligand-mediated superassembly technique is explored to assemble monodispersed, uneven, hole, open Au-silica (SiO2) nanotadpoles (AHOASTs). By manipulating the spatial density of ligands, the diploma of diffusion of silica might be exactly modulated; thus the diameters of the cavity might be constantly tuned. As a consequence of their extremely anisotropic, hole, open morphologies, we assemble a multicompartment nanocontainer with enzymes held and remoted contained in the cavity. Moreover, the ensuing enzyme–AHOASTs are used as biocompatible good H2O2-sensitive nanoswimmers and show a better diffusion coefficient than different nanoscaled swimmers. We imagine that this technique is vital not solely in designing refined hole nanosystem but additionally in offering nice alternatives for functions in nanomaterial meeting, catalysis, sensors, and nanoreactors.